- Discussion:
- muscle contraction is initiated w/ release of ionic calcium, from terminal cisternae into the sarcoplasm;
- complexing of calcium by troponin shifts the protein conformations in thin filament & allows formation of crossbridges between actin &
myosin, which then moves the actin molecule up long axis of the myosin filament;
- thick & thin filaments comes from cross-bridges undergo a conformational change which end up pulling thin filaments past the thick
filaments;
- as long as the calcium concentration in the cell is maintained in range of 10-6 M, the myosin ATPase remains active permiting the fibril to
stay in its contracted form;
- when energizing impulse terminates, Ca is rapidly pumped out of sarcoplasm;
- as ionic concentration drops, calcium is withdrawn from troponin, and when sufficiently low, the ATPase on myosin is inactivated;
- the troponin-actin-tropomyosin B complex again impedes the crossbridging between actin and myosin