Recombinant human bone morphogenetic protein induces bone formation
Wang-E-A. et. al. Genetics Institute, Cambridge, MA 02140. Proc-Natl-Acad-Sci-U-S-A. 1990 Mar. 87(6). P 2220-4. We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic [1mbone [m formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and [1mbone [m formation by day 14. The time at which [1mbone [m formation occurred was dependent on the amount of BMP-2A implanted; at high doses [1mbone [m formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo [1mbone [m formation in humans.
Original Text by Clifford R. Wheeless, III, MD.
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